ABSTRACT
To explore the function of a heat shock transcription factor gene (HSFB1) and its promoter in Amorphophallus, a 1 365 bp DNA sequence was obtained by homologous cloning from Amorphophallus albus. The gene expression level of AaHSFB1 determined by qRT-PCR indicated that AaHSFB1 gene is more sensitive to heat stress. The expression level of AaHSFB1 in roots increased followed by a decrease upon heat treatment, and the highest expression level was observed after heat treatment for 1 h. The expression level of AaHSFB1 in leaves reached the highest after heat treatment for 12 h. The expression level in bulbs did not change greatly during the heat treatment. Subcellular localization analysis showed that AaHSFB1 protein was localized in the nucleus. A 1 509 bp DNA sequence which contains the AaHSFB1 promoter was obtained by FPNI-PCR method. Bioinformatics analysis showed that the promoter contained heat stress response elements HSE and a plurality of cis-acting elements related to plant development and stress response. A prAaHSFB1::GUS fusion expression vector was constructed to further analyze the function of AaHSFB1 promoter. The expression vector was transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated method, and GUS staining analysis on transgenic plants after heat treatment was performed. The results showed that AaHSFB1 promoter had very high activity in the leaves. Therefore, we speculate that AaHSFB1 may play an important role in the stress resistance of A. albus, especially when encountering heat stress.
Subject(s)
Amorphophallus/metabolism , Arabidopsis/genetics , Cloning, Molecular , Gene Expression Regulation, Plant , Plant Proteins/metabolism , Plants, Genetically Modified/geneticsABSTRACT
With the constant change of global climate, plants are often affected by multiple abiotic stresses such as heat stress, drought stress, cold stress and saline-alkali stress. Heat shock transcription factors (HSFs) are a class of transcription factors widely existing in plants to respond to a variety of abiotic stresses. In this article, we review and summarize the structure, signal regulation mechanism of HSFs and some research in plants like Arabidopsis thaliana, tomato, rice and soybean, to provide reference for further elucidating the role of HSFs in the stress regulation network.
Subject(s)
Arabidopsis/metabolism , Droughts , Gene Expression Regulation, Plant , Heat Shock Transcription Factors/genetics , Plant Proteins/genetics , Stress, Physiological , Transcription Factors/metabolismABSTRACT
Objective@#To compare the efficacy of laparoscopic surgery with combined medial and caudal approach and cephalic medial-to-lateral approach for right hemicolon cancer patients combined with incomplete ileus.@*Methods@#The clinical data of 98 right hemicolon cancer (T1-4 M0) patients combined with incomplete ileus in Fukuang General Hospital of Liaoning Health Industry Group from January 2014 to December 2018 were retrospectively analyzed. All patients underwent laparoscopic right hemicolectomy, complete mesocolic excision (CME) and D3 lymphadenectomy. Among them, the combined medial and caudal approach was used in 50 patients (observation group), and the cephalic medial-to-lateral approach was used in 48 patients (control group). The operation time, bleeding volume, condition of lymph nodes dissected, length of hospital stay after operation and complication after operation were compared between 2 groups.@*Results@#Both groups successfully completed the operation, with no conversion to laparotomy and perioperative death. The bleeding volume and operation time in observation group were significantly lower than those in control group: (105.3 ± 22.6) ml vs. (309.6 ± 28.0) ml and (165.2 ± 17.9) min vs. (219.5 ± 21.5) min, and there were statistical differences (P<0.01 or <0.05). There were no statistical differences in the number of lymph nodes dissected, proportion of lymph nodes dissected ≥ 12, number of positive lymph, length of hospital stay after operation and complication after operation between 2 groups (P>0.05).@*Conclusions@#Laparoscopic surgery for right hemicolon cancer patients combined with incomplete ileus is safe and feasible. Compared with the cephalic medial-to-lateral approach, the combined medial and caudal approach can reduce the amount of intraoperative bleeding and shorten the operation time significantly.
ABSTRACT
Objective To compare the efficacy of laparoscopic surgery with combined medial and caudal approach and cephalic medial-to-lateral approach for right hemicolon cancer patients combined with incomplete ileus. Methods The clinical data of 98 right hemicolon cancer (T1- 4 M0) patients combined with incomplete ileus in Fukuang General Hospital of Liaoning Health Industry Group from January 2014 to December 2018 were retrospectively analyzed. All patients underwent laparoscopic right hemicolectomy, complete mesocolic excision (CME) and D3 lymphadenectomy. Among them, the combined medial and caudal approach was used in 50 patients (observation group), and the cephalic medial-to-lateral approach was used in 48 patients (control group). The operation time, bleeding volume, condition of lymph nodes dissected, length of hospital stay after operation and complication after operation were compared between 2 groups. Results Both groups successfully completed the operation, with no conversion to laparotomy and perioperative death. The bleeding volume and operation time in observation group were significantly lower than those in control group: (105.3 ± 22.6) ml vs. (309.6 ± 28.0) ml and (165.2 ± 17.9) min vs. (219.5 ± 21.5) min, and there were statistical differences (P﹤0.01 or ﹤0.05). There were no statistical differences in the number of lymph nodes dissected, proportion of lymph nodes dissected ≥ 12, number of positive lymph, length of hospital stay after operation and complication after operation between 2 groups (P＞0.05). Conclusions Laparoscopic surgery for right hemicolon cancer patients combined with incomplete ileus is safe and feasible. Compared with the cephalic medial-to-lateral approach, the combined medial and caudal approach can reduce the amount of intraoperative bleeding and shorten the operation time significantly.
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Objective To analyze the clinical value of percutaneous transhepatic biliary drainage (PTBD) in the treatment of obstructive jaundice.Methods A total of 479 patients with obstructive jaundice were enrolled in PLA General Hospital from July 2015 to July 2017 underwent percutaneous transhepatic biliary drainage,the preoperative serum total bilirubin was 92 ～ 614 μmol/L,the target bile duct was 2 ～ 16 mm in diameter.The PTBD was ultrasound guided.Results All of the 479 patients underwent PTBD successfully,and 99.6％ showed evident alleviation of jaundice postoperatively.Conclusions Ultrasoundguided PTBD is an effective method for reducing jaundice for preoperative or palliative treatment in patients with obstructive jaundice.The method of operation is worthy of further study.
ABSTRACT
Paget's disease of the breast is an uncommon disorder that accounts for 1% to 3% of all mammary tumors. The incidence of underlying carcinoma associated with Paget's disease has been reported in 82% to 100% of cases. The finding of underlying carcinoma reaches almost 100% when a palpable lump is also present. In this rare case, we described a patient presenting with Paget's disease but no palpable lump. However, we found 11 independent regions which were all invasive ductal carcinoma after the operation. Considering this patient, we should pay more attention to a multifocal and multicentric breast carcinoma associated with Paget's disease. Furthermore, we believe the mammography examination and a modified radical mastectomy are the most appropriate treatments for this population in clinical practice.